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one hundred µl HeLa nuclear extract (7 milligrams/ml), that was waiting since the before described [66], is actually pre-removed having streptavidin-decorated magnetized beans (Dynabeads, Invitrogen), mixed with 10 µl denatured Age. The new beads have been following wash 5 times by the incubation which have five hundred µl of just one× binding barrier (0.1% TritonX-100, 600 mM NaCl, 5 mM MgCl2, 1 mM DTT; Figure 3E). The new beads have been then remote, treated with ten µl from RNase services (0.1% TritonX-one hundred, 100 mM NaCl, step one mg/ml RNase A great, Sigma) to own 15 minute in the room temperature.

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